Detection of Intracellular Target Substances Using Raman Scattering Light

An important approach to identifying valuable E. gracilis involves detecting and visualizing whether the desired substances are present within cells and, if so, to what extent. While immunocytochemistry and staining reagents can label target substances within specific cells, methods requiring cell fixation are unsuitable for detecting intracellular target substances in live cells. Achieving this capability could facilitate real-time analysis of cells and the isolation of cells possessing target substances while alive, potentially leading to the acquisition of valuable cell strains. By employing labeling for cell surface antigens or staining reagents designed for l